The article is devoted to the analysis of causes for low entrepreneurial activity in Russia and its limited growth rates. The authors note that this situation is also typical for other transition countries and it is connected with historical background and mental peculiarities the absence thereof adaptive entrepreneurial culture. There are certain tendencies in development of mental characteristics of modern Russian business connected with certain features of national business environment. In-frastructural constituents of external business environment mold those standards, norms, rules that characterize entrepreneurial culture shape. Modern economic environment demands maximum efficiency of infrastructure facilities use for business support as a an input tool for business activity development, creation of an assessment system for evaluating the impact of support institutions on shaping positive properties of business environment. This paper highlights systematizing of infrastructural instruments of business activity support upon criterion of an impact extent on entrepreneurial culture condition. To such criteria the authors refer the following: attitude toward property; resource management; attitude toward own and hired human capital; attitude toward income and principles of its distribution; attitude toward social corporate responsibility; implemented business ethics principles. As a result, the authors reveal correlation of institutional tools application principles for additive entrepreneurial culture formation at different lifecycle milestones.